説明
The dataset consists of registrations of various species of helminths inhabiting bodies of representatives of common herpetofauna species of Ukraine. The dataset is a part of the project "Creating of genetic base of helminths from common species of amphibians and reptiles from the territory of Ukraine" № 29/02-2022(4) funded by National Academy of Sciences of Ukraine aimed on support of young scientists.
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、440 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Svitin R, Syrota Y, Kuzmin Y, Marushchak O, Dmytrieva I, Nechai A, Lisitsyna O (2023). Particular records of helminths from common species of herpetofauna of Ukraine. Version 1.8. Ukrainian Nature Conservation Group (NGO). Occurrence dataset. https://ukraine.ipt.gbif.no/resource?r=helminthsukraine2022&v=1.8
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は Ukrainian Nature Conservation Group (NGO)。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: ec73e150-38ce-46ff-9268-70bc0dd86a60が割り当てられています。 Participant Node Managers Committee によって承認されたデータ パブリッシャーとして GBIF に登録されているUkrainian Nature Conservation Group (NGO) が、このリソースをパブリッシュしました。
キーワード
Occurrence; Nematoda; Trematoda; Acantocephala; helminths; parasites; endoparasites; herpetofauna; dissections; Ukraine; biodiversity; null; Observation
連絡先
- 最初のデータ採集者
https://orcid.org/0000-0002-5450-7240
- 最初のデータ採集者
- 最初のデータ採集者
- メタデータ提供者 ●
- Custodiansteward(保管者） ●
- 最初のデータ採集者 ●
- 連絡先
- 最初のデータ採集者
- 最初のデータ採集者
- 最初のデータ採集者
地理的範囲
The dataset represents records of helminths made on the entire territory of Ukraine.
| 座標（緯度経度） | 南 西 [44.402, 22.17], 北 東 [52.376, 40.188] |
|---|
生物分類学的範囲
Dataset consists of records of 47 helminths' species from 3 most represented phylums: Nematoda, Acantocephala and Platyhelminthes. The parasites were excluded from representatives of 15 species of herpetofauna of Ukraine (8 from Amphibia class and 7 from Reptilia class).
| Kingdom | Animalia |
|---|---|
| Phylum | Nematoda, Acanthocephala, Platyhelminthes |
| Class | Chromadorea, Cestoda, Palaeacanthocephala, Monogenea, Trematoda |
| Order | Echinorhynchida, Onchoproteocephalidea, Polystomatidea, Diplostomida, Plagiorchiida, Polymorphida, Rhabditida, Dioctophymatida, Spirurida, Cyclophyllidea |
| Superfamily | Metastrongyloidea |
| Family | Acuariidae, Anisakidae, Centrorhynchidae, Cosmocercidae, Dioctophymatidae, Diplodiscidae, Diplostomidae, Echinorhynchidae, Encyclometridae, Gnathostomatidae, Haematoloechidae, Kathlanidae, Leptophallidae, Macroderidae, Mesocestoididae, Molineidae, Onchocercidae, Opisthorchiidae, Pharyngodonidae, Plagiorchiidae, Pleurogenidae, Polystomatidae, Proteocephalidae, Rhabdiasidae, Strigeidae, Strongyloididae, Telorchiidae |
時間的範囲
| 生成(収集）期間 | 2021/2023 |
|---|
プロジェクトデータ
The aims of the projects include: - collecting material from the maximum possible number of species of amphibians and reptiles from different regions of Ukraine; - identifying the collected types of parasites using light microscopy methods; - obtain the genetic sequences of the most widely used nuclear (18S) and mitochondrial (soh1).
| タイトル | Creating of genetic base of helminths from common species of amphibians and reptiles from the territory of Ukraine |
|---|---|
| 識別子 | 29/02-2022(4) |
| ファンデイング | The project is funded by National Academy of Sciences of Ukraine and is done in the I. I. Schmalhausen institute of zoology NAS of Ukraine. |
| Study Area Description | The projects covers the entire territory of Ukraine and includes the invasive study of communities of helminth presented in the target studied species of herpetofauna. |
| 研究の意図、目的、背景など（デザイン） | The projects is designed by young scientists. |
プロジェクトに携わる要員:
収集方法
During the reporting period, a number of expedition trips were made to various regions of Ukraine to collect material. The most favorable period for catching amphibians is the spring period, when they gather in large groups during spawning. But due to active hostilities and the ban on visiting forests and water bodies, most of the hosts for this project were collected in the period from the end of April to the beginning of May and until the beginning of November. This period is not critical, since the degree of helminth infection of the hosts increases due to active feeding, movement and completion of the spawning period (parasites that have entered the bodies of the hosts in the early larval stages have enough time to develop into adult stages) compared to the period immediately after wintering and under the time of spawning, when the hosts actively hardly eat, and individuals weakened by hyperinvasion of parasites died during wintering. During the warm period of 2022 and 2023 a total of 205 amphibians and reptiles were captured and dissected from Zakarpattia, Lviv, Ivano-Frankivsk, Poltava Zhytomyr, Vinnytsia, Odesa, Cherkasy, Kyiv regions and the city of Kyiv. These hosts included anuran amphibians: 23 grass frogs Rana temporaria L., 3 moor frogs R. arvalis (Nilsson, 1842), 12 common toads Bufo bufo L., 22 green toads Bufotes viridis (Laurenti, 1768), 12 common spadefoots Pelobates fuscus (Laurenti, 1768), 6 marsh frogs Pelophylax ridibundus (Pallas, 1771), 5 eastern tree frogs Hyla orientalis Bedriaga, 1890, 7 European fire-bellied toads Bombina bombina (L.) and 1 common newt Lissotriton vulgaris (L.); and reptiles: 23 sand lizards Lacerta agilis L., 21 viviparous lizards Zootoca vivipara Lichtenstein, 1823, 6 eastern slowworms Anguis colchica (Nordmann, 1840), 23 grass snakes Natrix natrix L., 7 dice snakes N. tessellata (Laurenti, 1768), 27 European mud turtles Emys orbicularis (L.) and 7 common vipers Vipera berus (L.). The material was collected from various regions of Ukraine, including Zaporizhzhia, Zhytomyr, Lviv, Vinnytsia, Cherkasy, Zakarpattia, Ivano-Frankivsk, Poltava, Odesa, Dnipropetrovsk, Kyiv regions and the city of Kyiv. Most of amphibians and reptiles were caught by hand or with a net (modified hydrobiological net or strong fishing net) and several terrapins were captured in traps. The turtle trap used – was the modified crab trap (with wider openings and attached plastic container for the bait). The most effective bait was freshly euthanised fish caught in the same water. Autopsies were performed in field laboratories directly during expedition trips, or live animals were transported to the laboratory of the I. I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine. Plastic containers of suitable sizes with holes for ventilation and lined with moist plant substrate, paper towels or soft cloth were used to transport the hosts. Amphibians and reptiles were euthanised by the injection of 10% lidocaine in the brain or bloodstream (terrapins) and dissected. For amphibians, the spinal cord was cut and the spinal canal was washed with saline using the thin Pasteur pipette in order to check for the presence of trematode larvae. This could not be done for reptile parasites due to thin spinal canal as it is too narrow and long. All organs were removed, washed in saline and checked for presence of parasites. The body cavity, large muscles and subcutaneous space was also checked for parasites in selected individuals. Found parasites were carefully removed, placed in small Petri dishes with saline and then fixed accordingly to the taxonomic group. Nematodes and smaller trematodes were fixed with hot 70% ethanol, monogeneans, large trematodes and cestodes were killed with hot water and then fixed with 70% ethanol, acanthocephalans were left in distilled water for up to 24 hours for the proboscis extending and then fixed with 70% ethanol. For larval stages found in cysts, part was excysted and fixed with 70% ethanol, while other part was fixed in cysts or (in case of hyper invasion with one species) counted and not taken. All parasites were transferred to vials with 70% ethanol ad subsequently stored in the fridge. Several monogeneans were fixed in 10% formalin and stored at room temperature. Trematodes were stained with Mayer's hematoxylin in the following stages: soaking the material extracted from 70% ethanol in water (5 min.); immersion in dye (5–10 min., the duration depends on the size of the individuals); immersion in a solution of hydrochloric acid (5 min.); ammonia solution (5 min.); passing through alcohols of increasing concentration (70%, 80%, 90%, 96%, 100% – 5(7) min. for each stage); enlightenment in clove oil (10–15 min.) and inclusion in Canadian balm on a permanent preparation. Dyeing with carmine was carried out according to the following scheme: immersion in dye (5–10 min.); acidified alcohol (5 min.); passing through alcohols of increasing concentration (80%, 90%, 96%, 100% - 5(7) minutes for each stage); enlightenment in clove oil (10–15 min.) and inclusion in Canadian balm on a permanent preparation. Research on the morphology of helminths was carried out under an AmScope T690B light microscope with a digital camera, and photomicrographs were obtained on a ZEISS Axio Imager M1 System microscope at the Center for Collective Use of Scientific Instruments "Animalia" (https://www.izan.kiev.ua/cen-coll.htm ) at the Institute of Zoology. The original and latest redescriptions of species descriptions were used for the determination, as well as the tables given in the monographs of K. M. Ryzhikov with co-authors (Рыжиков, 1980), V. P. Sharpila and N. I. Iskova (Шарпило, 1989) and in separate articles . Some individuals of the larval stages of nematodes and trematodes are defined only to higher taxonomic ranks, because they lack diagnostic features of taxa of a lower rank.
| Study Extent | Out of about 100 species of parasites known for amphibians and reptiles of Ukraine, the authors of this project collected 47 species in preliminary studies. All species were studied by light microscopy methods, but molecular genetic studies were not conducted on the territory of Ukraine. Overall, genetic sequences are available for less than 10% of parasite species, and there are less than 50 points of registrations for these representatives of the world's fauna highlighted for Ukraine. |
|---|---|
| Quality Control | The authors of the dataset are fully responsible for the quality of data provided in it: accuracy of identification, counting etc. |
Method step description:
- Conducting of field trips in search for the helminths' hosts, possibly infected by the target parasites.
- Humane autopsies of the hosts for the following dissections and study of helminths.
- Extracting and identification of helminths according to the standard methods.
- Georeferencing
- Organizing of the dataset according to the Darwin Core standards.
追加のメタデータ
| 代替識別子 | ec73e150-38ce-46ff-9268-70bc0dd86a60 |
|---|---|
| https://ukraine.ipt.gbif.no/resource?r=helminthsukraine2022 |